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Development and characterization of cannabidiol-loaded alginate copper hydrogel for repairing open bone defects in vitro

The clinical treatment of open bone defects caused by accidental bone trauma, bone tumors, bone diseases and bone infections is challenging. In this study, we designed and fabricated a multifunctional alginate-based hydrogel that contains cannabidiol (CBD), [email protected]/CBD hydrogel, for repairing open bone defects. The results of physicochemical characterization showed that the [email protected]/CBD hydrogel was successfully prepared and showed a suitable swelling ratio, high thermal stability, and stable mechanical properties. In vitro evaluation of antibacterial activity indicated that more than 90% of S. aureus and E. coli were inhibited compared to the control group. The ALP activity assay showed that the ALP expression level of MC3T3-E1cells in [email protected]/CBD hydrogel was approximately 2-fold higher than that in the control group on day 7 and 14. Additionally, compared to the control group, the level of mineralized deposits in [email protected]/CBD hydrogel was also improved by about 2 times on day 14. The PCR results indicated the mRNA expression levels of osteogenic markers (ALP, Col1?1, OCN, and RUNX2 genes) and angiogenic markers (EGFL6 and VEGF genes) in [email protected]/CBD hydrogel were significantly upregulated compared to that in the control group, and the mRNA expression levels of critical inflammatory cytokines (TNF-? and IL-1?) in the [email protected]/CBD hydrogel were significantly down-regulated compared to that in [email protected] hydrogel. Taken together, these results demonstrated that the [email protected]/CBD hydrogel showed significantly anti-bacterial, anti-inflammation, angiogenic and osteogenic activities in vitro studies. Thus, [email protected]/CBD hydrogels may be a promising candidate in repairing open bone defects.

» Author: Jianchao Qi, Zhe Zheng, Liqiu Hu, Huizhen Wang, Bin Tang, Lijun Lin

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