In this section, you can access to the latest technical information related to the FUTURE project topic.

Resveratrol and celastrol loaded Collagen dental implants regulate periodontal ligament fibroblast growth and osteoclastogenesis of bone marrow macrophages

Collagen is widely used for dental therapy in several ways such as films, 3D matrix, and composites, besides traditional Chinese medicine (TCM) has been used in tissue regeneration and wound healing application for centuries. Hence, the present study was targeted for the first time to fabricate collagen film with TCM such as resveratrol and celastrol in order to investigate the human periodontal ligament fibroblasts (HPLF) growth and bone marrow macrophages (BMM) derived osteoclastogenesis. Further, the physicochemical, mechanical and biological activities of collagen?TCMfilms crosslinked by glycerol and EDC?NHS (1?ethyl?3?(3?dimethyl aminopropyl)carbodiimide?N?hydroxysulfosuccinimide) were investigated. Collagen film characterization was significantly regulated by the nature of plasticizers like hydrophobic and degree of polarity. Interestingly, the collagen film?sdenaturation temperature was increased by EDC?NHS than glycerol. FTIR data confirmed the functional group changes due to chemical interaction of collagen with TCM. Morphological changes of HPLF cells cultured in control and collagen films were observed by SEM. Importantly, the addition of resveratrol upregulated the proliferation of HPLF cells, while osteoclastogenesis of BMM cells treated with mCSF?RANKL was significantly downregulated by celastrol. Accordingly, the collagen?TCMfilm could be an interesting material for dental regeneration especially it?s a therapeutic target to restrain the elevated bone resorption during osteoporosis.

» Author: Wenhui Wu, Ruijie Wang, Bin Bao, Chunling Bao, Shujun Wang, Saeed Ur Rahman, Chunyu Hou, Jeevithan Elango

» Reference: doi:10.1002/cbdv.202000295

» Publication Date: 10/07/2020

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