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Open fermentative production of fuel ethanol from food waste by an acid-tolerant mutant strain of Zymomonas mobilis

Publication date: March 2016
Source:Bioresource Technology, Volume 203

Author(s): Kedong Ma, Zhiyong Ruan, Zongxia Shui, Yanwei Wang, Guoquan Hu, Mingxiong He

The aim of present study was to develop a process for open ethanol fermentation from food waste using an acid-tolerant mutant of Zymomonas mobilis (ZMA7-2). The mutant showed strong tolerance to acid condition of food waste hydrolysate and high ethanol production performance. By optimizing fermentation parameters, ethanol fermentation with initial glucose concentration of 200g/L, pH value around 4.0, inoculum size of 10% and without nutrient addition was considered as best conditions. Moreover, the potential of bench scales fermentation and cell reusability was also examined. The fermentation in bench scales (44h) was faster than flask scale (48h), and the maximum ethanol concentration and ethanol yield (99.78g/L, 0.50g/g) higher than that of flask scale (98.31g/L, 0.49g/g). In addition, the stable cell growth and ethanol production profile in five cycles successive fermentation was observed, indicating the mutant was suitable for industrial ethanol production.





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