In this section, you can access to the latest technical information related to the FUTURE project topic.

Correction: Lentivirus-Mediated Knockdown of Astrocyte Elevated Gene-1 Inhibits Growth and Induces Apoptosis through MAPK Pathways in Human Retinoblastoma Cells

There is an error in the text of the Methods section in [1] reporting the primers used for RT-PCR. The correct text is: The primers for RT-PCR were: human AEG-1 (111bp): 5?-AAGCAGTGCAAAACAGTTCACG-3? forward, 5?-GCACCTTATCACGTTTACGCT-3? reverse; human GADPH (121bp): 5?-TGACTTCAACAGCGACACCCA-3? forward, 5?-CACCCTGTTGCTGTAGCCAAA-3? reverse.

The authors provide the following additional methodological information: We collected 54 clinical samples of retinoblastoma from the Ophthalmic Department of Beijing Tongren Hospital from March to December 2012. All tissues were fixed by 10% formaldehyde (volume fraction), embedded by conventional paraffin, stained by Haematoxylin-Eosin. The selected participants were not treated with chemotherapy or radiotherapy before surgery, and spontaneous retrograde atrophy eyeball was excluded. According to International Retinoblastoma Classification, the participants were divided into clinical phase D and phase E [2].

Fig 5A and 5B each incorrectly show a single shared GAPDH panel for the western blot experiments. The western blot for each protein was carried out with its own GAPDH loading control. A revised Fig 5 is provided in which the correct corresponding GAPDH loading control panels from the original experiment are shown. Please see the correct Fig 5 here.

» Author: Ying Chang,  Bin Li,  Xiaolin Xu,  Ling Shen,  Haixia Bai,  Fei Gao,  Zhibao Zhang,  Jost B. Jonas

» Reference: https://doi.org/10.1371/journal.pone.0223818

» Publication Date: 14/10/2019

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