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Overproduction of bioactive algal chrysolaminarin by the critical carbon flux regulator phosphoglucomutase

AbstractChrysolaminarin, the primary polysaccharide reservoir in some marine algae, has attracted much attention due to its broad health properties. However, its biosynthetic pathway and regulation mechanisms have rarely been reported which hinders the improvement of production efficiency. Therefore, in this study, we aimed to identify key metabolic nodes in the chrysolaminarin biosynthetic pathway. We identified a phosphoglucomutase (PGM) in the model microalga Phaeodactylum tricornutum, and revealed its critical role in chrysolaminarin biosynthesis. PGM overexpression significantly elevated chrysolaminarin content by 2.54?fold and reached 25.6% of cell dry weight, while algal growth and photosynthesis were not impaired. Besides, PGM overexpression up? and down? regulated the expression of chrysolaminarin and lipid biosynthetic genes, respectively. Microscopic analysis of aniline blue stained cells revealed that overproduced chrysolaminarin localized predominantly in vacuoles. Lipidomic analyses revealed that PGM overexpression significantly reduced the lipid content. The findings reveal the critical role of phosphoglucomutase in regulating the carbon flux between carbohydrate and lipid biosynthesis in microalgae, and provide a promising candidate for high efficiency production of chrysolaminarin.

» Author: Yu?Feng Yang , Da?Wei Li , Ting?Ting Chen , Ting?Bin Hao , Srinivasan Balamurugan , Wei?Dong Yang , Jie?Sheng Liu , Hong?Ye Li

» Reference: doi:10.1002/biot.201800220

» Publication Date: 04/08/2018

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