In this section, you can access to the latest technical information related to the FUTURE project topic.

3-D magnetic graphene oxide-magnetite poly(vinyl alcohol) nanocomposite substrates for immobilizing enzyme

Three-dimensional magnetic graphene oxide-magnetite polyvinyl alcohol (3D-GO/PVA/Fe3O4) nanocomposites were successfully prepared. The morphology was characterized and analyzed through scanning electron microscope (SEM) and transmission electron microscope (TEM). The chemical structure and the crystal structure were explored by X-ray powder diffraction (XPS), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction spectra (XRD). The magnetic property was obtained by vibrating sample magnetometer (VSM). The specific surface area and the average pore size were determined by Brunauer-Emmett-Teller (BET) and Barrett–Joyner–Halenda (BJH). The specific surface and the average pore size of 3D-GO/PVA/Fe3O4 nanocomposites were 388.87 m2g-1 and 9.6?nm, and the higher specific surface indicated that the three-dimensional structure avoided the aggregation for GO sheets. The large saturation magnetization (Ms) of the nanocomposites of 30.5 emu/g enabled the easy cycling of the nanocomposites. The 3D-GO/PVA/Fe3O4 nanocomposites exhibited better performance for porcine pancreatic lipase (PPL) enzyme immobilization. The maximum immobilization efficiency was 91%, and the enzyme immobilized 3D-GO/PVA/Fe3O4 nanocomposites reached up to 90% of their activities. After 10 cycles of reuse, the activity of immobilized enzyme remained about 70.8% of the initial activity. The stability test revealed that the activity of immobilized enzyme remained up to 71.1% at 4?°C for 56 days.

» Author: Yanyun Li, Tao Jing, Gaofeng Xu, Jingzhi Tian, Mengyao Dong, Qian Shao, Bin Wang, Zhikang Wang, Yongjie Zheng, Changlong Yang, Zhanhu Guo

» Reference: Polymer, Volume 149

» Publication Date: 01/08/2018

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