In this section, you can access to the latest technical information related to the FUTURE project topic.

Microcontact printing of polydopamine on thermally expandable hydrogels for controlled cell adhesion and delivery of geometrically defined microtissues

Scaffold-free harvest of microtissue with a defined structure has received a great deal of interest in cell-based assay and regenerative medicine. In this study, we developed thermally expandable hydrogels with spatially controlled cell adhesive patterns for rapid harvest of geometrically controlled microtissue. We patterned polydopamine (PD) on to the hydrogel via microcontact printing (?CP), in linear shapes with widths of 50, 100 and 200?m. The hydrogels facilitated formation of spatially controlled strip-like microtissue of human dermal fibroblasts (HDFBs). It was possible to harvest and translocate microtissues with controlled widths of 61.4±14.7, 104.3±15.6, and 186.6±22.3?m from the hydrogel to glass substrates by conformal contact upon expansion of the hydrogel in response to a temperature change from 37 to 4°C, preserving high viability, extracellular matrix, and junction proteins. Microtissues were readily translocated in vivo to the subcutaneous tissue of mouse. The microtissues were further utilized as a simple assay model for monitoring of contraction in response to ROCK1 inhibitor. Collectively, micro-sized patterning of PD on the thermally expandable hydrogels via ?CP holds promise for the development of microtissue harvesting systems that can be employed to ex vivo tissue assay and cell-based therapy.

» Author: Yu Bin Lee, Se-jeong Kim, Eum Mi Kim, Hayeon Byun, Hyung-kwan Chang, Jungyul Park, Yu Suk Choi, Heungsoo Shin

» Reference: Acta Biomaterialia, Volume 61

» Publication Date: 01/10/2017

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