In this section, you can access to the latest technical information related to the FUTURE project topic.

Synthesis and binding affinity analysis of ?1-2- and ?1-6-O/S-linked dimannosides for the elucidation of sulfur in glycosidic bonds using quartz crystal microbalance sensors

The role of sulfur in glycosidic bonds has been evaluated using quartz crystal microbalance methodology. Synthetic routes towards ?1-2- and ?1-6-linked dimannosides with S- or O-glycosidic bonds have been developed, and the recognition properties assessed in competition binding assays with the cognate lectin concanavalin A. Mannose-presenting QCM sensors were produced using photoinitiated, nitrene-mediated immobilization methods, and the subsequent binding study was performed in an automated flow-through instrumentation, and correlated with data from isothermal titration calorimetry. The recorded K d-values corresponded well with reported binding affinities for the O-linked dimannosides with affinities for the ?1-2-linked dimannosides in the lower micromolar range. The S-linked analogs showed slightly disparate effects, where the ?1-6-linked analog showed weaker affinity than the O-linked dimannoside, as well as positive apparent cooperativity, whereas the ?1-2-analog displayed very similar binding compared to the O-linked structure.

» Author: Oscar Norberg, Bin Wu, Niranjan Thota, Jiantao Ge, Germain Fauquet, Ann-Kathrin Saur, Teodor Aastrup, Hai Dong, Mingdi Yan, Olof Ramström

» Reference: Carbohydrate Research

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